The research work presented in this book was carried out using the world's oldest single-Dee variable energy Cyclotron (VEC), installed at Panjab University in Chandigarh, India.

This classic 26-inch VEC is capable of delivering beams of 1H, 2H, 3H, and 4He. For the past fifteen years, the facility has been extensively used for Ion Beam Analysis (IBA) employing a proton beam.

Recently, the facility has been standardized for the simultaneous analysis of X-rays and backscattered ions from biological samples. The advantages of using coupled ion beam techniques for comprehensive elemental profiling of organometallic samples are unmatched.

Thus, the standardization will facilitate the interaction of metal ions with metalloproteins studied using Particle Induced X-ray Emission (PIXE) spectroscopy and Proton Elastic Scattering Analysis (PESA) simultaneously. Beam discharge calibration has been performed to reliably estimate the total number of incident projectile ions.